rat igg2a isotype control Search Results


isotype control abs  (R&D Systems)
94
R&D Systems isotype control abs
Isotype Control Abs, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rat monoclonal igg2a isotype control  (Novus Biologicals)
99
Novus Biologicals rat monoclonal igg2a isotype control
Rat Monoclonal Igg2a Isotype Control, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rat igg 2a isotype  (R&D Systems)
94
R&D Systems rat igg 2a isotype
Rat Igg 2a Isotype, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rat immunoglobulin g2a igg2a isotype control mab  (R&D Systems)
95
R&D Systems rat immunoglobulin g2a igg2a isotype control mab
FIG. 4. Effects of Cs (A) or anti-IFN-g MAb (B) on LPS-induced serum TNF-a activity in BALB/c-AnNCr mice primed with TSST-1. Groups of four mice were pretreated with Cs (40 mg/kg, i.p.) or anti-IFN-g MAb (750 mg/mouse, i.p.) at 4 or 2 h, respectively, prior to injection of TSST-1. Mice were then injected i.p. with 200 mg of TSST-1 (dotted bars) per kg or PBS (clear bars). Control mice received equal volumes of PBS instead of Cs, rat <t>IgG2a</t> instead of anti-IFN-g MAb, or PBS instead of TSST-1. All mice were injected with LPS (400 mg/kg, i.p.) 12 h later. TNF-a activity in serum was measured 2 h postinjection of LPS. Solutions of Cs or MAb were treated with 2 mg of polymyxin B per ml to remove contaminating LPS. The percent reductions in circulating TNF-a activity caused by Cs or anti-IFN-g MAb are shown.
Rat Immunoglobulin G2a Igg2a Isotype Control Mab, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sca 1  (R&D Systems)
90
R&D Systems sca 1
FIG. 4. Effects of Cs (A) or anti-IFN-g MAb (B) on LPS-induced serum TNF-a activity in BALB/c-AnNCr mice primed with TSST-1. Groups of four mice were pretreated with Cs (40 mg/kg, i.p.) or anti-IFN-g MAb (750 mg/mouse, i.p.) at 4 or 2 h, respectively, prior to injection of TSST-1. Mice were then injected i.p. with 200 mg of TSST-1 (dotted bars) per kg or PBS (clear bars). Control mice received equal volumes of PBS instead of Cs, rat <t>IgG2a</t> instead of anti-IFN-g MAb, or PBS instead of TSST-1. All mice were injected with LPS (400 mg/kg, i.p.) 12 h later. TNF-a activity in serum was measured 2 h postinjection of LPS. Solutions of Cs or MAb were treated with 2 mg of polymyxin B per ml to remove contaminating LPS. The percent reductions in circulating TNF-a activity caused by Cs or anti-IFN-g MAb are shown.
Sca 1, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rat igg2a isotype control antibody  (MedChemExpress)
94
MedChemExpress rat igg2a isotype control antibody
FIG. 4. Effects of Cs (A) or anti-IFN-g MAb (B) on LPS-induced serum TNF-a activity in BALB/c-AnNCr mice primed with TSST-1. Groups of four mice were pretreated with Cs (40 mg/kg, i.p.) or anti-IFN-g MAb (750 mg/mouse, i.p.) at 4 or 2 h, respectively, prior to injection of TSST-1. Mice were then injected i.p. with 200 mg of TSST-1 (dotted bars) per kg or PBS (clear bars). Control mice received equal volumes of PBS instead of Cs, rat <t>IgG2a</t> instead of anti-IFN-g MAb, or PBS instead of TSST-1. All mice were injected with LPS (400 mg/kg, i.p.) 12 h later. TNF-a activity in serum was measured 2 h postinjection of LPS. Solutions of Cs or MAb were treated with 2 mg of polymyxin B per ml to remove contaminating LPS. The percent reductions in circulating TNF-a activity caused by Cs or anti-IFN-g MAb are shown.
Rat Igg2a Isotype Control Antibody, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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2i inhibitors  (Miltenyi Biotec)
94
Miltenyi Biotec 2i inhibitors
FIG. 4. Effects of Cs (A) or anti-IFN-g MAb (B) on LPS-induced serum TNF-a activity in BALB/c-AnNCr mice primed with TSST-1. Groups of four mice were pretreated with Cs (40 mg/kg, i.p.) or anti-IFN-g MAb (750 mg/mouse, i.p.) at 4 or 2 h, respectively, prior to injection of TSST-1. Mice were then injected i.p. with 200 mg of TSST-1 (dotted bars) per kg or PBS (clear bars). Control mice received equal volumes of PBS instead of Cs, rat <t>IgG2a</t> instead of anti-IFN-g MAb, or PBS instead of TSST-1. All mice were injected with LPS (400 mg/kg, i.p.) 12 h later. TNF-a activity in serum was measured 2 h postinjection of LPS. Solutions of Cs or MAb were treated with 2 mg of polymyxin B per ml to remove contaminating LPS. The percent reductions in circulating TNF-a activity caused by Cs or anti-IFN-g MAb are shown.
2i Inhibitors, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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isotype control  (Miltenyi Biotec)
94
Miltenyi Biotec isotype control
FIG. 4. Effects of Cs (A) or anti-IFN-g MAb (B) on LPS-induced serum TNF-a activity in BALB/c-AnNCr mice primed with TSST-1. Groups of four mice were pretreated with Cs (40 mg/kg, i.p.) or anti-IFN-g MAb (750 mg/mouse, i.p.) at 4 or 2 h, respectively, prior to injection of TSST-1. Mice were then injected i.p. with 200 mg of TSST-1 (dotted bars) per kg or PBS (clear bars). Control mice received equal volumes of PBS instead of Cs, rat <t>IgG2a</t> instead of anti-IFN-g MAb, or PBS instead of TSST-1. All mice were injected with LPS (400 mg/kg, i.p.) 12 h later. TNF-a activity in serum was measured 2 h postinjection of LPS. Solutions of Cs or MAb were treated with 2 mg of polymyxin B per ml to remove contaminating LPS. The percent reductions in circulating TNF-a activity caused by Cs or anti-IFN-g MAb are shown.
Isotype Control, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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isotype control rat anti-igg2a percp  (Miltenyi Biotec)
94
Miltenyi Biotec isotype control rat anti-igg2a percp
FIG. 4. Effects of Cs (A) or anti-IFN-g MAb (B) on LPS-induced serum TNF-a activity in BALB/c-AnNCr mice primed with TSST-1. Groups of four mice were pretreated with Cs (40 mg/kg, i.p.) or anti-IFN-g MAb (750 mg/mouse, i.p.) at 4 or 2 h, respectively, prior to injection of TSST-1. Mice were then injected i.p. with 200 mg of TSST-1 (dotted bars) per kg or PBS (clear bars). Control mice received equal volumes of PBS instead of Cs, rat <t>IgG2a</t> instead of anti-IFN-g MAb, or PBS instead of TSST-1. All mice were injected with LPS (400 mg/kg, i.p.) 12 h later. TNF-a activity in serum was measured 2 h postinjection of LPS. Solutions of Cs or MAb were treated with 2 mg of polymyxin B per ml to remove contaminating LPS. The percent reductions in circulating TNF-a activity caused by Cs or anti-IFN-g MAb are shown.
Isotype Control Rat Anti Igg2a Percp, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rat igg2a  (Biogems International)
90
Biogems International rat igg2a
Figure 5. TANs drive B-cell differentiation independently of T cells. A, Quantification of CD138 expression on splenic B220þ cells following various culture conditions—when cultured alone (B), with T cells (B þ T, ratio 1:1), with TANs (B þ TAN, ratio 1:1), with TAN and T cells (B þ TAN þ T, ratio 1:1:1), with T cells but no contact allowed with TANs (B þT//TAN,ratio 1:1:1), or TANs cocultured with T cells but no contact allowed with B cells (B//T þTAN, ratio 1:1:1). Data represent the mean SEM (n ¼ 6–9; , P < 0.001; n.s., not significant). Groups were compared using one-way ANOVA. B, Representative flow plots displaying CD138 expression out of total B220þ population in the different coculture conditions. C, Quantification of <t>IgG</t> release to the media following overnight coculture of isolated splenic B220þ
Rat Igg2a, supplied by Biogems International, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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igg2a fitc isotype control antibody  (Miltenyi Biotec)
94
Miltenyi Biotec igg2a fitc isotype control antibody
Figure 5. TANs drive B-cell differentiation independently of T cells. A, Quantification of CD138 expression on splenic B220þ cells following various culture conditions—when cultured alone (B), with T cells (B þ T, ratio 1:1), with TANs (B þ TAN, ratio 1:1), with TAN and T cells (B þ TAN þ T, ratio 1:1:1), with T cells but no contact allowed with TANs (B þT//TAN,ratio 1:1:1), or TANs cocultured with T cells but no contact allowed with B cells (B//T þTAN, ratio 1:1:1). Data represent the mean SEM (n ¼ 6–9; , P < 0.001; n.s., not significant). Groups were compared using one-way ANOVA. B, Representative flow plots displaying CD138 expression out of total B220þ population in the different coculture conditions. C, Quantification of <t>IgG</t> release to the media following overnight coculture of isolated splenic B220þ
Igg2a Fitc Isotype Control Antibody, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti igg isotype control  (R&D Systems)
93
R&D Systems anti igg isotype control
Figure 5. TANs drive B-cell differentiation independently of T cells. A, Quantification of CD138 expression on splenic B220þ cells following various culture conditions—when cultured alone (B), with T cells (B þ T, ratio 1:1), with TANs (B þ TAN, ratio 1:1), with TAN and T cells (B þ TAN þ T, ratio 1:1:1), with T cells but no contact allowed with TANs (B þT//TAN,ratio 1:1:1), or TANs cocultured with T cells but no contact allowed with B cells (B//T þTAN, ratio 1:1:1). Data represent the mean SEM (n ¼ 6–9; , P < 0.001; n.s., not significant). Groups were compared using one-way ANOVA. B, Representative flow plots displaying CD138 expression out of total B220þ population in the different coculture conditions. C, Quantification of <t>IgG</t> release to the media following overnight coculture of isolated splenic B220þ
Anti Igg Isotype Control, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


FIG. 4. Effects of Cs (A) or anti-IFN-g MAb (B) on LPS-induced serum TNF-a activity in BALB/c-AnNCr mice primed with TSST-1. Groups of four mice were pretreated with Cs (40 mg/kg, i.p.) or anti-IFN-g MAb (750 mg/mouse, i.p.) at 4 or 2 h, respectively, prior to injection of TSST-1. Mice were then injected i.p. with 200 mg of TSST-1 (dotted bars) per kg or PBS (clear bars). Control mice received equal volumes of PBS instead of Cs, rat IgG2a instead of anti-IFN-g MAb, or PBS instead of TSST-1. All mice were injected with LPS (400 mg/kg, i.p.) 12 h later. TNF-a activity in serum was measured 2 h postinjection of LPS. Solutions of Cs or MAb were treated with 2 mg of polymyxin B per ml to remove contaminating LPS. The percent reductions in circulating TNF-a activity caused by Cs or anti-IFN-g MAb are shown.

Journal: Infection and Immunity

Article Title: Role of T Cells and Gamma Interferon during Induction of Hypersensitivity to Lipopolysaccharide by Toxic Shock Syndrome Toxin 1 in Mice

doi: 10.1128/iai.69.3.1256-1264.2001

Figure Lengend Snippet: FIG. 4. Effects of Cs (A) or anti-IFN-g MAb (B) on LPS-induced serum TNF-a activity in BALB/c-AnNCr mice primed with TSST-1. Groups of four mice were pretreated with Cs (40 mg/kg, i.p.) or anti-IFN-g MAb (750 mg/mouse, i.p.) at 4 or 2 h, respectively, prior to injection of TSST-1. Mice were then injected i.p. with 200 mg of TSST-1 (dotted bars) per kg or PBS (clear bars). Control mice received equal volumes of PBS instead of Cs, rat IgG2a instead of anti-IFN-g MAb, or PBS instead of TSST-1. All mice were injected with LPS (400 mg/kg, i.p.) 12 h later. TNF-a activity in serum was measured 2 h postinjection of LPS. Solutions of Cs or MAb were treated with 2 mg of polymyxin B per ml to remove contaminating LPS. The percent reductions in circulating TNF-a activity caused by Cs or anti-IFN-g MAb are shown.

Article Snippet: Neutralizing rat monoclonal antibody (MAb) to mouse IFN-g, rat immunoglobulin G2a (IgG2a) isotype control MAb, and murine recombinant TNF-a were obtained from R&D Systems (Minneapolis, Minn.).

Techniques: Activity Assay, Injection, Control

Figure 5. TANs drive B-cell differentiation independently of T cells. A, Quantification of CD138 expression on splenic B220þ cells following various culture conditions—when cultured alone (B), with T cells (B þ T, ratio 1:1), with TANs (B þ TAN, ratio 1:1), with TAN and T cells (B þ TAN þ T, ratio 1:1:1), with T cells but no contact allowed with TANs (B þT//TAN,ratio 1:1:1), or TANs cocultured with T cells but no contact allowed with B cells (B//T þTAN, ratio 1:1:1). Data represent the mean SEM (n ¼ 6–9; , P < 0.001; n.s., not significant). Groups were compared using one-way ANOVA. B, Representative flow plots displaying CD138 expression out of total B220þ population in the different coculture conditions. C, Quantification of IgG release to the media following overnight coculture of isolated splenic B220þ

Journal: Cancer Immunology Research

Article Title: Tumor-Associated Neutrophils Drive B-cell Recruitment and Their Differentiation to Plasma Cells

doi: 10.1158/2326-6066.cir-20-0839

Figure Lengend Snippet: Figure 5. TANs drive B-cell differentiation independently of T cells. A, Quantification of CD138 expression on splenic B220þ cells following various culture conditions—when cultured alone (B), with T cells (B þ T, ratio 1:1), with TANs (B þ TAN, ratio 1:1), with TAN and T cells (B þ TAN þ T, ratio 1:1:1), with T cells but no contact allowed with TANs (B þT//TAN,ratio 1:1:1), or TANs cocultured with T cells but no contact allowed with B cells (B//T þTAN, ratio 1:1:1). Data represent the mean SEM (n ¼ 6–9; , P < 0.001; n.s., not significant). Groups were compared using one-way ANOVA. B, Representative flow plots displaying CD138 expression out of total B220þ population in the different coculture conditions. C, Quantification of IgG release to the media following overnight coculture of isolated splenic B220þ

Article Snippet: Isotype control antibodies were as follows: APCconjugated rat IgG2a (clone 2A3; Biogems), FITC-conjugated rat IgG2b (clone RTK4530; BioLegend), BGViolet450 rat IgG2a (clone 2A3, Biogems), PE-conjugated rat IgG2a (clone 2A3, Biogems).

Techniques: Cell Differentiation, Expressing, Cell Culture, Isolation

Figure 6. TANs express membranal BAFF, but not membranal APRIL, and mediate B-cell IgG production in a BAFF-R–dependent manner. A and B, Expression of membranal BAFF and APRIL in TANs within the whole tumor (A) and following TANs' isolation from the tumors (B). Representative histograms showing BAFF and APRIL expression in TANs (gated as total Ly6Gþ population) are provided (right plots). C, IgG production by splenic B cells cocultured with TANs (ratio 1:5) in the absence or presence of anti–BAFF-R antibody. Data represent the mean SEM (n ¼ 4; , P < 0.01; , P < 0.001). Groups were compared using one-way ANOVA. D, Quantification of CD138 expression on isolated splenic B220þ cells cultured alone or cocultured with TANs (ratio 1:5), without or with blocking of the three potential BAFF receptors, BAFF-R, TACI, or BCMA. Data represent the mean SEM (n ¼ 4–10; , P < 0.001; n.s., nonsignificant). Groups were compared using one-way ANOVA.

Journal: Cancer Immunology Research

Article Title: Tumor-Associated Neutrophils Drive B-cell Recruitment and Their Differentiation to Plasma Cells

doi: 10.1158/2326-6066.cir-20-0839

Figure Lengend Snippet: Figure 6. TANs express membranal BAFF, but not membranal APRIL, and mediate B-cell IgG production in a BAFF-R–dependent manner. A and B, Expression of membranal BAFF and APRIL in TANs within the whole tumor (A) and following TANs' isolation from the tumors (B). Representative histograms showing BAFF and APRIL expression in TANs (gated as total Ly6Gþ population) are provided (right plots). C, IgG production by splenic B cells cocultured with TANs (ratio 1:5) in the absence or presence of anti–BAFF-R antibody. Data represent the mean SEM (n ¼ 4; , P < 0.01; , P < 0.001). Groups were compared using one-way ANOVA. D, Quantification of CD138 expression on isolated splenic B220þ cells cultured alone or cocultured with TANs (ratio 1:5), without or with blocking of the three potential BAFF receptors, BAFF-R, TACI, or BCMA. Data represent the mean SEM (n ¼ 4–10; , P < 0.001; n.s., nonsignificant). Groups were compared using one-way ANOVA.

Article Snippet: Isotype control antibodies were as follows: APCconjugated rat IgG2a (clone 2A3; Biogems), FITC-conjugated rat IgG2b (clone RTK4530; BioLegend), BGViolet450 rat IgG2a (clone 2A3, Biogems), PE-conjugated rat IgG2a (clone 2A3, Biogems).

Techniques: Expressing, Isolation, Cell Culture, Blocking Assay